probdnf antibody (Alomone Labs)
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Probdnf Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/probdnf/Anti-proBDNF+Antibody/pmc13187084-83-26-28
Average 94 stars, based on 42 article reviews
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1) Product Images from "Long non-coding SINEUP RNA enhancing BDNF translation prevents dendritic atrophy following an Aβ-peptide challenge"
Article Title: Long non-coding SINEUP RNA enhancing BDNF translation prevents dendritic atrophy following an Aβ-peptide challenge
Journal: Cellular and Molecular Life Sciences: CMLS
doi: 10.1007/s00018-026-06175-4
Figure Legend Snippet: SINEUP strategy, specificity and efficacy in targeting BDNF protein upregulation. A-B . Rat BDNF gene structure with schematic representation of BDNF transcripts characterised by multiple alternative splicing of 5’ and 3’UTRs (grey boxes), a coding sequence (CDS, white boxes) and two poly-adenylation sites (polyA). B. SINEUP-PAN and SINEUP-Ex1 annealing sites on BDNF transcripts used in this study. C-H. HEK293T cells are co-transfected with different plasmids to demonstrate the efficacy of SINEUP-PAN or SINEUP-Ex1 on the indicated BDNF transcripts with respect to control (GFP). Plasmids were transfected in 1:3 ratio. Tubulin (55 kDa) is indicative of gel loading and is used for normalization in the Western blot analysis. Histograms quantify average data from n = 4 experiments. The molecular weight for the BDNF-GFP band of 64–65 KDa correspond to the predicted size for a chimeric protein made of the proBDNF form (32–34 KDa) and the GFP (27–30 KDa). C . Effect of SINEUP-PAN on protein levels encoded by the transfected chimeric Ex1-BDNF-GFP transcript with respect to control represented by the plasmid encoding the reporter protein GFP alone. p = 0.0005. D . Effect of SINEUP-Ex1 on protein levels encoded by the transfected chimeric Ex1-BDNF-GFP transcript. p = 0.0299. E-F . Effect of SINEUP-PAN on protein levels encoded by the transfected chimeric Ex2B-BDNF-GFP (E) and chimeric Ex2C-BDNF-GFP (F) transcripts. n = 3. G-H . Effect of SINEUP-Ex1 on chimeric Ex2B-BDNF-GFP (G) and chimeric Ex2C-BDNF-GFP (H) transcripts with respect to control (GFP). All the quantitative results shown are produced from a repetition of n = 3 experiments and the Western blot images show the greatest effect obtained and therefore, are not representative of the average results
Techniques Used: Alternative Splicing, Sequencing, Transfection, Control, Western Blot, Molecular Weight, Plasmid Preparation, Produced
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